|
| Status |
Public on Feb 27, 2023 |
| Title |
cpsf160 ChIP in control sample repeat 2 |
| Sample type |
SRA |
| |
|
| Source name |
cpsf160 ChIP in control sample repeat 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
transfection: control
|
| Treatment protocol |
control and U1 AMOs were transfected into HeLa cells.
|
| Growth protocol |
Hela cells were grown in DMEM +10% FBS medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seqs were carried out using standard protocol
library were prepared using ChIP-seq preparation kit (Vazyme)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
ChIP-seq data processing and analysis were performed according to the ENCODE ChIP-seq pipeline (https://www.encodeproject.org/data-standards/chip-seq/)
Genome_build: hg38
Supplementary_files_format_and_content: Read coverage tracks are generated in the BigWig (bw) format from the genome-mapped bam file.
|
| |
|
| Submission date |
Jan 07, 2022 |
| Last update date |
Feb 27, 2023 |
| Contact name |
chengguo yao |
| E-mail(s) |
yaochguo@mail.sysu.edu.cn
|
| Organization name |
sysu
|
| Department |
school of medicine
|
| Street address |
No. 74 zhongshan 2rd road
|
| City |
guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE192943 |
The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs |
| GSE193202 |
ChIP-seq analysis for core 3' processing factors in control and U1 AMO-treated HeLa cells |
|
| Relations |
| BioSample |
SAMN24720035 |
| SRA |
SRX13652954 |
