|
Status |
Public on Feb 27, 2023 |
Title |
cpsf160 ChIP in U1 AMO sample repeat 2 |
Sample type |
SRA |
|
|
Source name |
cpsf160 ChIP in U1 AMO sample repeat 2
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa transfection: U1 AMO
|
Treatment protocol |
control and U1 AMOs were transfected into HeLa cells.
|
Growth protocol |
Hela cells were grown in DMEM +10% FBS medium
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP-seqs were carried out using standard protocol library were prepared using ChIP-seq preparation kit (Vazyme)
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
ChIP-seq data processing and analysis were performed according to the ENCODE ChIP-seq pipeline (https://www.encodeproject.org/data-standards/chip-seq/) Genome_build: hg38 Supplementary_files_format_and_content: Read coverage tracks are generated in the BigWig (bw) format from the genome-mapped bam file.
|
|
|
Submission date |
Jan 07, 2022 |
Last update date |
Feb 27, 2023 |
Contact name |
chengguo yao |
E-mail(s) |
yaochguo@mail.sysu.edu.cn
|
Organization name |
sysu
|
Department |
school of medicine
|
Street address |
No. 74 zhongshan 2rd road
|
City |
guangzhou |
ZIP/Postal code |
510080 |
Country |
China |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE192943 |
The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs |
GSE193202 |
ChIP-seq analysis for core 3' processing factors in control and U1 AMO-treated HeLa cells |
|
Relations |
BioSample |
SAMN24720033 |
SRA |
SRX13652956 |