|
| Status |
Public on Mar 01, 2011 |
| Title |
Naïve tumor day 30, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
SKOV-3 xenograft tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: xenograft tumor
cell line: SKOV-3
treatment: vehicle
|
| Treatment protocol |
Tumors were removed from mice and quickly frozen in liquid nitrogen.
|
| Growth protocol |
SKOV-3 cells were suspended in matrigel and inoculated subcutaneously into nude mice for two weeks before treatments were initiated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol and Qiagen RNAsy was performed following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 300ng total RNA (GeneChip Whole Transcript (WT) Sense Target Labeling Assay Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, of about 2.3 ug cRNA were hybridized for 17 hr at 45C on each GeneST 1.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using fluidics script FS450_007
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G scanner equipped with an autoloader.
|
| Description |
Gene expression data from vehicle treated SKOV-3 xenograft after 30 days
|
| Data processing |
The data were analyzed with plier-gcbg from Affymetrix Power Tools using HuGene-1_0-st-v1.r3.bgp, HuGene-1_0-st-v1.r3.pgf, and HuGene-1_0-st-v1.r3.clf f iles from Affymetrix.
|
| |
|
| Submission date |
Aug 13, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Alexander Brodsky |
| E-mail(s) |
alex_brodsky@brown.edu
|
| Phone |
401-444-1649
|
| Organization name |
Rhode Island Hospital/Brown University
|
| Department |
MCB
|
| Lab |
Brodsky lab
|
| Street address |
593 Eddy St.
|
| City |
PROVIDENCE |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE23616 |
Integrated Genomics of Ovarian Xenograft Tumor Progression and Chemotherapy Response |
|
