|
| Status |
Public on Nov 14, 2022 |
| Title |
MALE ischemic mice #4 - CL |
| Sample type |
RNA |
| |
|
| Source name |
mouse brain, healthy hemisphere (CL)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6j
age: 8-12 weeks
condition: young
Sex: male
treatment: ischemic
tissue: brain
brain hemisphere: CL
|
| Treatment protocol |
flash frozen at -80ºC
|
| Growth protocol |
Saline-perfused mouse brain samples were isolated; IP and CL hemispheres were separated and flash frozen with liquid N2 until RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from frozen brain samples with the MirVana™ Paris™ (Thermo Fisher Scientific Inc., USA) and kept at -80ºC until the array was performed.
|
| Label |
biotin
|
| Label protocol |
Samples were amplified and labeled using GeneChip WT Plus Reagent Kit
|
| |
|
| Hybridization protocol |
cDNA was hybridizated in GeneChip Mouse Clariom S Affymetrix microarrays for 16 hours with rotation at 45 ºC with rotation
|
| Scan protocol |
Microarrays were scanned in Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from mouse brain
|
| Data processing |
the images were processed with the Affymetrix GeneChip Command Console software for CEL file generation. To check the array image quality, Affymetrix Expression Console was used. Samples that demonstrated good quality results (pm mean, bgrd mean, pos vs neg auc,...) were subjected to subsequent analysis
|
| |
|
| Submission date |
Feb 07, 2022 |
| Last update date |
Nov 14, 2022 |
| Contact name |
Laura Ramiro |
| E-mail(s) |
laura.ramirop@gmail.com, laura.ramiro@vhir.org
|
| Organization name |
Vall d'Hebron Institute of Research
|
| Lab |
Neurovascular research group
|
| Street address |
Passeig De La Vall D'hebrón 119-129, Edificio Mediterránea, Laboratorio 120
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08035 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24242 |
| Series (1) |
| GSE196266 |
Expression data from the mouse brain after 90'-MCAO and sham-control surgery in: young male mice, young female mice, diabetic young male mice and aged male mice |
|
