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Sample GSM5866694 Query DataSets for GSM5866694
Status Public on Nov 14, 2022
Title MALE ischemic mice #6 - IP
Sample type RNA
 
Source name mouse brain, ischemic hemisphere (IP)
Organism Mus musculus
Characteristics strain: C57Bl/6j
age: 8-12 weeks
condition: young
Sex: male
treatment: ischemic
tissue: brain
brain hemisphere: IP
Treatment protocol flash frozen at -80ºC
Growth protocol Saline-perfused mouse brain samples were isolated; IP and CL hemispheres were separated and flash frozen with liquid N2 until RNA isolation.
Extracted molecule total RNA
Extraction protocol RNA was isolated from frozen brain samples with the MirVana™ Paris™ (Thermo Fisher Scientific Inc., USA) and kept at -80ºC until the array was performed.
Label biotin
Label protocol Samples were amplified and labeled using GeneChip WT Plus Reagent Kit
 
Hybridization protocol cDNA was hybridizated in GeneChip Mouse Clariom S Affymetrix microarrays for 16 hours with rotation at 45 ºC with rotation
Scan protocol Microarrays were scanned in Affymetrix GeneChip Scanner 3000
Description Gene expression data from mouse brain
Data processing the images were processed with the Affymetrix GeneChip Command Console software for CEL file generation. To check the array image quality, Affymetrix Expression Console was used. Samples that demonstrated good quality results (pm mean, bgrd mean, pos vs neg auc,...) were subjected to subsequent analysis
 
Submission date Feb 07, 2022
Last update date Nov 14, 2022
Contact name Laura Ramiro
E-mail(s) laura.ramirop@gmail.com, laura.ramiro@vhir.org
Organization name Vall d'Hebron Institute of Research
Lab Neurovascular research group
Street address Passeig De La Vall D'hebrón 119-129, Edificio Mediterránea, Laboratorio 120
City Barcelona
State/province Barcelona
ZIP/Postal code 08035
Country Spain
 
Platform ID GPL24242
Series (1)
GSE196266 Expression data from the mouse brain after 90'-MCAO and sham-control surgery in: young male mice, young female mice, diabetic young male mice and aged male mice

Data table header descriptions
ID_REF
VALUE log2RMA mean signal estimates

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.095680852
AFFX-BioB-5_at 7.140944587
AFFX-BioB-M_at 7.915142118
AFFX-BioC-3_at 7.691423402
AFFX-BioC-5_at 8.507621976
AFFX-BioDn-3_at 10.37069686
AFFX-BioDn-5_at 9.296728776
AFFX-BkGr-GC03_st 1.368004649
AFFX-BkGr-GC04_st 1.126119193
AFFX-BkGr-GC05_st 1.11076123
AFFX-BkGr-GC06_st 1.168470865
AFFX-BkGr-GC07_st 1.180162465
AFFX-BkGr-GC08_st 1.174363185
AFFX-BkGr-GC09_st 1.268233641
AFFX-BkGr-GC10_st 1.38337735
AFFX-BkGr-GC11_st 1.419228499
AFFX-BkGr-GC12_st 1.516547768
AFFX-BkGr-GC13_st 1.687969799
AFFX-BkGr-GC14_st 1.773177746
AFFX-BkGr-GC15_st 1.974053194

Total number of rows: 29129

Table truncated, full table size 818 Kbytes.




Supplementary file Size Download File type/resource
GSM5866694_G05_15BT_IP.CEL.gz 1013.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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