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Status |
Public on Nov 24, 2010 |
Title |
8688T1 tumor treated, grade3 |
Sample type |
RNA |
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Source name |
8688T1 tumor treated, grade3
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Organism |
Mus musculus |
Characteristics |
strain: C57/B6-129SvJae tissue: lung tumor gender: female
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Treatment protocol |
For treated samples tamoxifen was administered IP in 2 injections. Tumors were collected 7 days after first injection.
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Growth protocol |
Tumors were extrracted from 10 week old animals
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cDNA was prepared from 100 ng total RNA using Nugen Ovation RNA amplification system V2 according to the manufacturers instructions.
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Hybridization protocol |
Following fragmentation, 4 ug of cDNA was hybridized for 16 hr at 45C on Mouse 430A_2 chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the affymetrix 3000 7G scanner.
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Description |
Gene expression data from microdissected p53 restored adenocarcinomas
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Data processing |
R version 2.11.0 (x86_64-pc-linux-gnu), affyPLM_1.24.0, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.0, Biobase_2.8.0
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Submission date |
Aug 30, 2010 |
Last update date |
Nov 24, 2010 |
Contact name |
Charles Arthur Whittaker |
E-mail(s) |
charliew@mit.edu
|
Organization name |
Koch Institute
|
Street address |
77 Mass Ave 76-189
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02152 |
Country |
USA |
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|
Platform ID |
GPL8321 |
Series (2) |
GSE23874 |
Stage-specific sensitivity to p53 restoration in lung cancer: tumor data |
GSE23875 |
Stage-specific sensitivity to p53 restoration in lung cancer |
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