|
| Status |
Public on Sep 01, 2011 |
| Title |
14 |
| Sample type |
RNA |
| |
|
| Source name |
6 sets of 50 mid 3rd instar control larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Hml-Gal4>;UAS-GFP transgenic line
developmental stage: mid 3rd instar larvae
environmental treatment group: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Kit
|
| Label |
Biotin
|
| Label protocol |
Affymetrix One Cycle Target Labeling according to manufacturer's protocol (GeneChip¨ Expression Analysis Technical Manual)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol (GeneChip¨ Expression Analysis Technical Manual)
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip¨ Expression Analysis Technical Manual)
|
| Description |
6 sets of 50 mid 3rd instar control larvae
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Aug 30, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sharmila Bhattacharya |
| E-mail(s) |
sharmila.bhattacharya@nasa.gov
|
| Organization name |
NASA Ames Research Center
|
| Street address |
Bldg. 236, Room 213
|
| City |
Moffet Field |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE23880 |
Drosophila melanogaster gene expression changes after spaceflight. |
|
| Relations |
| Reanalyzed by |
GSE119084 |
