|
| Status |
Public on Jan 27, 2011 |
| Title |
Cell culture from patient11 treated with LY2109761 |
| Sample type |
RNA |
| |
|
| Source name |
Primary culture of human glioblatoma
|
| Organism |
Homo sapiens |
| Characteristics |
treatment group: treated with LY2109761
cell type: Primary culture of human glioblatoma
tumor type: Glioblastoma
|
| Treatment protocol |
Cells were treated with 2microM LY2109761 for 3 hours
|
| Growth protocol |
Patient derived cells were grown in culture in the presence of DMEM+10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was synthesized with the IVT Labeling kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
07SE210HGU133Plus_2.0_230207.CEL
Gene expression of primary culture glioblastoma
|
| Data processing |
The data were analyzed with GeneSpring GX v7.3
|
| |
|
| Submission date |
Sep 01, 2010 |
| Last update date |
Jan 27, 2011 |
| Contact name |
Joan Seoane |
| Organization name |
VHIO
|
| Street address |
Vall d'Hebron 119-129
|
| City |
Barcelona |
| ZIP/Postal code |
08029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23935 |
Gene responses to TGF-beta receptor inhibition in glioblastoma |
|
