|
| Status |
Public on Mar 31, 2022 |
| Title |
input2 |
| Sample type |
SRA |
| |
|
| Source name |
Input iTreg cells before aCD3 stimulation
|
| Organism |
Mus musculus |
| Characteristics |
cell type: sgRNA-transduced iTreg cells
strain: C57BL/6
|
| Treatment protocol |
iTreg cells were restimulated with 0.25 or 4 ug/ml aCD3 for 3 hrs
|
| Growth protocol |
Naïve CD4 T cells transduced with indicated sgRNA library were cultured with 2ng/ml TGFb and 10ng/ml IL-2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted with the 'DNeasy Blood & Tissue Kits' from Qiagen
The barcoding reaction was run using the NEBNext HiFi kit on the basis of the manufacturer’s instructions and amplified for five cycles
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Input iTreg cells before restimulation
|
| Data processing |
Library strategy: CRISPR screen
Adapter sequences were removed by mageck 0.5.9.4 software
and aligned them to the sgRNA sequence of Brie CRISPR library
Supplementary files format and content: sgRNA counts for all samples which includes sgRNA sequence and raw counts
|
| |
|
| Submission date |
Mar 30, 2022 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE160598 |
CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity |
| GSE199813 |
CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [CRISPR screen] |
|
| Relations |
| BioSample |
SAMN27112541 |
| SRA |
SRX14677303 |
