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Status
Public on Oct 08, 2010
Title
T.4.Pa.BDC#5
Sample type
RNA
Source name
pancreas
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Collagenase Type IV / DNase digestion, Flow sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
T.4.Pa.BDC#5, Pancreas-Infiltrating CD4, BDC islet-reactive TCR Tg
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
18.7184
10344622
226.243
10344624
432.5835
10344633
907.1799
10344637
227.6247
10344653
16.5026
10344658
276.8881
10344674
11.771
10344679
48.933
10344707
319.7672
10344713
459.2193
10344723
242.0032
10344725
43.4493
10344741
2822.0116
10344743
31.0888
10344750
253.9993
10344772
23.8207
10344789
214.5298
10344797
354.8866
10344799
253.7516
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605757_EA07068_58847_MoGene_T.4.Pa.BDC_#5.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
