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Sample GSM605792 Query DataSets for GSM605792
Status Public on Oct 08, 2010
Title Tgd.vg2+.Sp.TCRbko#3
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 5 weeks
exclusion markers: CD4- CD8- NK1.1-
genetic background: C57BL/6J
phenotype markers: TCRd+ Vg2+ CD44-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass Tissue Grinder, MACS depletion / Flow Sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Tgd.vg2+.Sp.TCRbko#3, spleen TCRd+ Vg2+ CD44-
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.6867
10344622 261.4088
10344624 298.0838
10344633 710.9504
10344637 178.4119
10344653 21.9106
10344658 187.3385
10344674 10.9222
10344679 47.0167
10344707 237.4112
10344713 420.5068
10344723 165.3729
10344725 37.9952
10344741 2382.8312
10344743 30.8612
10344750 273.1145
10344772 29.1527
10344789 119.2058
10344797 400.1327
10344799 180.2304

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605792_EA07068_54561_MoGene_Tgd.vg2+.Sp.TCRbko_#3.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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