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Sample GSM605875 Query DataSets for GSM605875
Status Public on Oct 08, 2010
Title Mo.6C+II-.LN#1
Sample type RNA
 
Source name Mesenteric LN
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: Nk1.1- B220-
genetic background: C57BL/6J
phenotype markers: F480+ Ly6c+ MHCII lo/+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Collagenase D, 30 min
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Mo.6C+II-.LN#1, Classical monocytes MHC II-
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 20.1082
10344622 335.0154
10344624 380.7611
10344633 631.4808
10344637 240.6681
10344653 13.0087
10344658 153.5954
10344674 11.3163
10344679 36.1757
10344707 114.8789
10344713 189.2425
10344723 161.3613
10344725 47.3507
10344741 1725.254
10344743 28.9139
10344750 977.9802
10344772 32.7764
10344789 87.4352
10344797 360.846
10344799 143.8837

Total number of rows: 24922

Table truncated, full table size 423 Kbytes.




Supplementary file Size Download File type/resource
GSM605875_EA07068_103577_MoGene_MO.6C+II-.LN#1.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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