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Sample GSM605877 Query DataSets for GSM605877
Status Public on Oct 08, 2010
Title Mo.6C+II-.LN#3
Sample type RNA
 
Source name Mesenteric LN
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: Nk1.1- B220-
genetic background: C57BL/6J
phenotype markers: F480+ Ly6c+ MHCII lo/+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Collagenase D, 30 min
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description Mo.6C+II-.LN#3, Classical monocytes MHC II-
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.8713
10344622 272.5999
10344624 441.0836
10344633 632.7017
10344637 269.4152
10344653 17.8285
10344658 204.3587
10344674 9.3579
10344679 43.0547
10344707 96.8678
10344713 238.4601
10344723 237.8138
10344725 47.2929
10344741 2027.5775
10344743 29.6571
10344750 1141.8413
10344772 39.5782
10344789 130.9954
10344797 285.332
10344799 189.5202

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605877_EA07068_103579_MoGene_MO.6C+II-.LN#3.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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