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Sample GSM605892 Query DataSets for GSM605892
Status Public on Oct 08, 2010
Title T.8Eff.Sp.OT1.d10.LisOva#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: B220- CD45.2- Gr-1- NK1.1- CD4- MHCII- P.I.-
genetic background: C57BL/6J
phenotype markers: CD8+ CD45.1+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol frosted microscope teasing
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8Eff.Sp.OT1.d10.LisOva#2, spleen OT1 tg effectors CD8 day 10 Listeria.OVA replicate 2
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 14.8359
10344622 245.9862
10344624 408.3764
10344633 916.696
10344637 190.3056
10344653 21.1338
10344658 305.2092
10344674 11.866
10344679 51.7167
10344707 348.0046
10344713 327.1686
10344723 107.6058
10344725 42.3544
10344741 2133.2311
10344743 38.6722
10344750 72.8864
10344772 23.4082
10344789 146.7428
10344797 343.2289
10344799 212.7652

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605892_EA07068_85511_MoGene_T.8EFF.SP.OT1.D10.LISOVA#2.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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