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Sample GSM605901 Query DataSets for GSM605901
Status Public on Oct 08, 2010
Title T.8Eff.Sp.OT1.d8.LisOva#1
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: B220- CD45.2- Gr-1- NK1.1- CD4- MHCII- P.I.-
genetic background: C57BL/6J
phenotype markers: CD8+ CD45.1+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol frosted microscope teasing
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.8Eff.Sp.OT1.d8.LisOva#1, spleen OT1 tg effectors CD8 day 8 Listeria.OVA
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Oct 07, 2010
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 19.0042
10344622 270.0753
10344624 430.3944
10344633 702.8348
10344637 243.579
10344653 14.1946
10344658 386.257
10344674 10.9928
10344679 39.1279
10344707 331.3978
10344713 504.5746
10344723 167.03
10344725 54.1151
10344741 1961.5099
10344743 33.7648
10344750 111.4149
10344772 23.2244
10344789 192.2167
10344797 354.9936
10344799 227.3063

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM605901_EA07068_82681_MoGene_T.8EFF.SP.OT1.D8LISO.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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