|
| Status |
Public on Oct 08, 2010 |
| Title |
T.8Mem.Sp.OT1.d100.LisOva#4 |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
age: 6 weeks
exclusion markers: B220- CD45.2- Gr-1- NK1.1- CD4- MHCII- P.I.-
genetic background: C57BL/6J
phenotype markers: CD8+ CD45.1+
separation method: dissection/trimming
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
frosted microscope teasing
|
| Label |
Biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
T.8Mem.Sp.OT1.d100.LisOva#4, spleen OT1 tg Memory CD8 day 100 Lis.OVA repliacate 4
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Oct 07, 2010 |
| Last update date |
May 24, 2016 |
| Contact name |
Liang Yang |
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
