|
Status |
Public on Mar 31, 2011 |
Title |
Control, doxcycline 24 h, rep 1 |
Sample type |
RNA |
|
|
Source name |
TW01Tet
|
Organism |
Homo sapiens |
Characteristics |
tissue: nasopharyngeal carcinoma cell line: TW01Tet immortalization method: EBV negative
|
Treatment protocol |
Cells were treated with doxycycline (50 nanogram/ml) for 24 h.
|
Growth protocol |
Cells were grown to log-phase in DMEM supplemented with 10% tetracycline-free fetal bovine serum before induction.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a RNeasy Mini kit (Qiagene), according to the manufacturer’s instructions. A 2-round amplification was performed with the ExpressArt TRinucleotide mRNA Amplification Kit (AmpTec) according to the manufacturer’s instructions.
|
Label |
biotin
|
Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
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|
|
Hybridization protocol |
Samples were hybridized with GeneChip Human Gene 1.0 ST Arrays (Affymetrix) and scanned at the NHRI Microarray Core Facility (Miaoli County, Taiwan).
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
Description |
Cell densitiy was maintained at 150,000 / ml before induction.
|
Data processing |
The data were analyzed using Affymetrix Expression Console™ Software (version 1.1) default analysis settings and RMA (Robust Multi-Array) summarization and normalization options.
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|
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Submission date |
Oct 07, 2010 |
Last update date |
Mar 31, 2011 |
Contact name |
Su-Fang Lin |
E-mail(s) |
sflin1@gmail.com
|
Organization name |
National Health Research Institutes, Taiwan
|
Department |
NICR
|
Lab |
R2-1211
|
Street address |
No 35, Keyan Road, Zhunan Town
|
City |
Miaoli Conunty |
ZIP/Postal code |
35053 |
Country |
Taiwan |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE24586 |
Expression profiling of host genes modulated by Epstein-Barr virus Rta in nasopharyngeal carcinoma cells |
GSE24587 |
Epstein–Barr virus (EBV) Rta-mediated cell cycle arrest enables permissive replication of EBV and Kaposi’s sarcoma-associated herpesvirus in 293 cells |
|