|
| Status |
Public on Oct 21, 2010 |
| Title |
mCG-PR_KLS_6wks_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: Bl/6 Taconic
genotype/variation: mCG-PR N17
tissue: Bone marrow
cell type: KLS
time of progression: 6 wks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA).Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
|
| Label |
biotin
|
| Label protocol |
Amplified cDNA was biotin labeled using the EncoreTM Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Labeled targets were hybridized to Mouse Exon 1.0ST arrays (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Gene chips were scanned using Affymetrix GeneArray scanner GS 3000.
|
| Description |
17263
|
| Data processing |
Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
|
| |
|
| Submission date |
Oct 15, 2010 |
| Last update date |
Oct 20, 2010 |
| Contact name |
Nobish Varghese |
| E-mail(s) |
nvarghese@path.wustl.edu
|
| Organization name |
Washington University in St.Louis
|
| Street address |
4444 Forest Park Ave.
|
| City |
StLouis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE24728 |
mCG-PML-RARA alters multipotent hematopoiesis. |
|
