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Status |
Public on Nov 26, 2010 |
Title |
M238_R1_B_DMSO |
Sample type |
RNA |
|
|
Source name |
Human melanoma cell lines
|
Organism |
Homo sapiens |
Characteristics |
cell type: Human melanoma cell line cell line: M238_R1 genotype: V600E BRAF mutant treatment group: DMSO control
|
Treatment protocol |
Cell lines were treated with DMSO control or 1 uM PLX4032 for 6h
|
Growth protocol |
Selected melanoma cell lines were grown in DMEM supplemented with 10% serum
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RiboPure Kit (Ambion) according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
cDNAs were generated from total RNA and biotinylated according to the standard Affymetrix protocol.
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|
|
Hybridization protocol |
Labeled cDNAs were hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix) according to the manufacturer's protocol
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Scan protocol |
Array scanning was carried out with the GeneChip Scanner 3000 7G (Affymetrix) according to the manufacturer's instructions
|
Data processing |
Expression data were normalized, background-corrected, and log2-transformed for parametric analysis. Differentially expressed genes were identified using significance analysis of microarrays (SAM) with the R package 'samr' (FDR<0.05; fold change>2)
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|
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Submission date |
Oct 21, 2010 |
Last update date |
Nov 26, 2010 |
Contact name |
Mikyung Lee |
Organization name |
UCLA
|
Street address |
UCLA Med-Derm BOX 951781, 13-942 Factor, 13-952 Factor Los Angeles, CA 90095-1781
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90024 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE24862 |
Expression data from melanoma cell lines |
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