|
| Status |
Public on Nov 26, 2010 |
| Title |
M249_B_PLX |
| Sample type |
RNA |
| |
|
| Source name |
Human melanoma cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human melanoma cell line
cell line: M249
genotype: V600E BRAF mutant
treatment group: 1 uM PLX4032 for 6h
|
| Treatment protocol |
Cell lines were treated with DMSO control or 1 uM PLX4032 for 6h
|
| Growth protocol |
Selected melanoma cell lines were grown in DMEM supplemented with 10% serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RiboPure Kit (Ambion) according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
cDNAs were generated from total RNA and biotinylated according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Labeled cDNAs were hybridized to the GeneChip Human Gene 1.0 ST Arrays (Affymetrix) according to the manufacturer's protocol
|
| Scan protocol |
Array scanning was carried out with the GeneChip Scanner 3000 7G (Affymetrix) according to the manufacturer's instructions
|
| Data processing |
Expression data were normalized, background-corrected, and log2-transformed for parametric analysis. Differentially expressed genes were identified using significance analysis of microarrays (SAM) with the R package 'samr' (FDR<0.05; fold change>2)
|
| |
|
| Submission date |
Oct 21, 2010 |
| Last update date |
Nov 26, 2010 |
| Contact name |
Mikyung Lee |
| Organization name |
UCLA
|
| Street address |
UCLA Med-Derm BOX 951781, 13-942 Factor, 13-952 Factor Los Angeles, CA 90095-1781
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90024 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE24862 |
Expression data from melanoma cell lines |
|
