|
| Status |
Public on Jul 05, 2022 |
| Title |
Ad-5XSRSF1-U1_Ad |
| Sample type |
SRA |
| |
|
| Source name |
In vitro transcribed RNA
|
| Organism |
Human adenovirus 2 |
| Characteristics |
tissue: In vitro transcribed RNA
cell line: N/A
cell type: N/A
genotype: Wild-type
|
| Treatment protocol |
In the Fastq file names, N indicates treatment with NMIA, D DMSO, DC denatured control
|
| Growth protocol |
In vitro transcribed RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purification by Monarch RNA cleanup kit
Two-step PCR
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
MiSeq Nano
|
| Data processing |
Basecalls performed with Realtime Analysis (RTA) software launched automatically by MiSeq Control Software
All steps of the processing were carried out by SHAPEMapper 2.0 pipeline
|
| |
|
| Submission date |
May 11, 2022 |
| Last update date |
Jul 06, 2022 |
| Contact name |
Kaushik Saha |
| E-mail(s) |
ksaha79@googlemail.com
|
| Organization name |
University of California San Diego
|
| Department |
Chemistry and Biochemistry
|
| Lab |
NSB 3325A
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0375 |
| Country |
USA |
| |
|
| Platform ID |
GPL30034 |
| Series (2) |
| GSE188652 |
Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly |
| GSE202785 |
[SHAPE-MaP_10_mM] U1 snRNP regulates recruitment of early spliceosomal components by disrupting SRSF1-pre-mRNA interactions |
|
| Relations |
| BioSample |
SAMN28194737 |
| SRA |
SRX15237125 |
