|
| Status |
Public on Oct 29, 2010 |
| Title |
PrtT-GC_1 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells treated with A. fumigatus PrtT null mutant germinating conidia, 8 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: alveolar epithelial cell line
cell line: A549
treatment group: treated with A. fumigatus PrtT null mutant germinating conidia, 8 h
|
| Treatment protocol |
Cells were incubated for 8 h with WT A. fumigatus conidia (AF293), ΔPrtT protease-deficient conidia (AF293 background), A. fumigatus CF, and ΔPrtT protease-deficient CF.
|
| Growth protocol |
A549 cells were grown at a density of 1 × 106 cells/100 mm dish, and cultured in serum-free minimal essential medium (MEM) for 16 to 18 h before treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from treated and control uninfected A549 cells with RNeasy columns (Qiagen, Hilden, Germany) as recommended by Affymetrix (Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse-transcribed (Agilent Technologies, Santa Clara, CA) and the resulting cDNA was used as a template for in-vitro transcription with biotin-labeled oligonucleotides.
|
| |
|
| Hybridization protocol |
Following fragmentation, labeled cDNA was hybridized to the array as directed by the manufacturer (Affymetrix).
|
| Scan protocol |
Scanning was performed by Gene Chip Scanner 3000 and images analyzed by imaging software GCOS v1
|
| Data processing |
The data were analyzed with Expreddion Console (RMA) using Affymetrix default analysis settings. Data was transpormed to log2 scale. Further Quantile normalization was executed using the Expander software
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
Oct 28, 2010 |
| Contact name |
Nir Osherov |
| E-mail(s) |
nosherov@post.tau.ac.il
|
| Phone |
972 3 640 9599
|
| Fax |
972 3 640 9160
|
| Organization name |
Tel-Aviv University
|
| Department |
Medical Microbiology and Immunology
|
| Lab |
Aspergillus Lab Osherov
|
| Street address |
Ramat Aviv Campus
|
| City |
Tel-Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE24984 |
Response of A549 cells treated with Aspergillus fumigatus [WT-GC_vs_PrtT-GC] |
| GSE24986 |
Response of A549 cells treated with Aspergillus fumigatus |
|
