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Status |
Public on Nov 01, 2011 |
Title |
(+)/loxMZ_rep3 |
Sample type |
RNA |
|
|
Source name |
(+)/loxMZ_rep3
|
Organism |
Mus musculus |
Characteristics |
strain: C57/Bl6, possibly with some contribution of CBA tissue: spleen cell type: marginal zone (MZ) B cells genotype/variation: CD19-Creki/+Dicerfl/+
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA of purified populations was extracted with TRIzol (Invitrogen) following manufacturer’s instructions.
|
Label |
Cy3
|
Label protocol |
Amount of nucleic acid labeled: 120ng. Commercial miRNA Microarray System with miRNA Complete Labeling and Hyb Kit by following manufacturer instructions. Agilent manual G4170-90011 Ver. 2.0 June 2008. Fluorescent Cyanine 3-pCp molecules are ligated with T4 RNA ligase to dephosphorilated 3' ends from the RNA sample.
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|
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Hybridization protocol |
Chamber type: SureHyb hybridization chamber (Agilent). Quantity of each labeled extract used: all the labeled sample. Duration: 40 hours. Volume: 45 uL. Temperature (ºC): 55.
|
Scan protocol |
Scanned on an G2565C DNA microarray scanner (Agilent). Images were quantified using Agilent Feature Extraction Software (ver. 10.1.1).
|
Description |
US22502553_251911910077_S01_miRNA-v1_95_May07_1_4.txt
|
Data processing |
Images were quantified and normalized using default normalization with Agilent Feature Extraction Software (ver. 10.1.1).
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|
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Submission date |
Nov 04, 2010 |
Last update date |
Nov 01, 2011 |
Contact name |
Almudena Ramiro |
E-mail(s) |
arodriguezr@cnio.es
|
Organization name |
CNIO
|
Street address |
Calle Melchor Fernandez Almagro 3
|
City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
|
|
Platform ID |
GPL8824 |
Series (1) |
GSE25139 |
miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells |
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