|
| Status |
Public on Dec 09, 2022 |
| Title |
40_ZSz |
| Sample type |
SRA |
| |
|
| Source name |
leaf
|
| Organism |
Malus domestica |
| Characteristics |
cultivar: cultivar Fuji
place of origin: Zalaszanto
year of sampling: 2015
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CTAB method (Gambino, 2008: https://doi.org/10.1002/pca.1078 )
modified Illumina Trueseq small RNA kit protocol: https://doi.org/10.1007/978-1-4939-7683-6_9
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
pooled RNA from leaves
|
| Data processing |
The quality check of the sequenced small RNA libraries was done by FastQC Program and CLC Genomic Workbench 11
Trimming of raw reads was performed using the CLC Genomic Workbench 11
Removing redundant reads using the CLC Genomic Workbench 11
Supplementary files format and content: trimmed redundant list, each read with its number of repeats
|
| |
|
| Submission date |
May 31, 2022 |
| Last update date |
Dec 09, 2022 |
| Contact name |
Eva Varallyay |
| E-mail(s) |
Varallyay.Eva@uni-mate.hu
|
| Organization name |
Hungarian University of Agriculture and Life Sciences
|
| Department |
Institute of Plant Protection
|
| Lab |
Department of Plant Pathology, Genomics Research group
|
| Street address |
4 Szent-Györgyi Albert Street
|
| City |
Godollo |
| ZIP/Postal code |
H-2100 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL18137 |
| Series (1) |
| GSE205183 |
New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens |
|
| Relations |
| BioSample |
SAMN28870840 |
| SRA |
SRX15597380 |
