|
| Status |
Public on Jun 17, 2022 |
| Title |
Control human umbilical vein endothelial cells |
| Sample type |
RNA |
| |
|
| Source name |
human umbilical vein endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human umbilical vein endothelial like cell line
tissue: umbilical vein
|
| Treatment protocol |
Images were quantified using Agilent Feature Extraction Software
|
| Growth protocol |
ECV304 cells were cultured in maintenance medium (MEM, Gibco, US) containing 10% fetal bovine serum, glutamine, sodium bicarbonate and penicillin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
4 µg of total RNA were primed with 5 µl of T7 DNA primer at 65°C for 10 min, then reversed transcribed at 40°C for 2 h in the presence of 1 µl MMLV, and 10 µM dNTP,0.1 M DTT, with 25 µM dCTP, 10 mM each Cy3-label and Cy5-label
|
| |
|
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
|
| Description |
Control ECV304 cells, untreated, harvested cultured cells on the 2nd, 4th and 6th day for experiment
|
| Data processing |
The chip was scanned in Agilent 2565BA gene chip scanner, and the scanned data were analyzed and homogenized by Feature Extraction software.
|
| |
|
| Submission date |
Jun 16, 2022 |
| Last update date |
Jun 17, 2022 |
| Contact name |
gong meiling |
| E-mail(s) |
gongmeiling@sina.com
|
| Organization name |
College of Life Sciences and Engineering
|
| Street address |
Foshan university
|
| City |
Foshan |
| ZIP/Postal code |
528225 |
| Country |
China |
| |
|
| Platform ID |
GPL887 |
| Series (1) |
| GSE206320 |
Human umbilical vein endothelial cells: Control vs. Uninfected Groups |
|
