|
| Status |
Public on Jun 29, 2023 |
| Title |
MCF7 cells, glucose starvation, Biol rep3 |
| Sample type |
SRA |
| |
|
| Source name |
MCF7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7 cells
cell type: epithelial breast cancer cell line
genotype: WT
treatment: Glucose withdraw
|
| Treatment protocol |
MCF7 cells are seeded on culture plate 2 days prior of experiment. On the day of the experiment, for the fast group, the cells were changed to fresh DMEM complete medium for 4h before treatment to ensure the viability. Then the cells were changed to glucose-free DMEM medium for 4 hours before collection. For the refed group, cells were also changed to fresh DMEM medium for 4h and followed by glucose-free DMEM medium for 4 hours. Then the cells were refed by changing to complete DMEM before collection.
|
| Growth protocol |
MCF7 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Invitrogen TRIzol according to manufacturer's instruction.
1.0 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Starvation3
|
| Data processing |
Clean reads were aligned to hg38 using HISAT2.
FeatureCounts was applied to quantify the number of reads mapped to each gene.
Assembly: hg38
Supplementary files format and content: raw_count_rs.csv: Comma-separated values file includes raw count for all the samples.
|
| |
|
| Submission date |
Jun 17, 2022 |
| Last update date |
Jun 29, 2023 |
| Contact name |
FengFeng Rao |
| E-mail(s) |
raof@sustech.edu.cn
|
| Organization name |
Southern University of Science and Technology
|
| Street address |
1088 Xueyuan Road
|
| City |
Shenzhen |
| State/province |
N/A |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE206394 |
Effect of glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells |
| GSE206396 |
Effect of short-term glucose withdrawal and glucose refeeding after short-term glucose withdrawal on gene expression of MCF7 cells |
|
| Relations |
| BioSample |
SAMN29180883 |
| SRA |
SRX15783364 |
