|
| Status |
Public on Dec 25, 2010 |
| Title |
Sorted positive cells at E17.5 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
BAC-Crx-EGFP-positive-retina
|
| Organism |
Mus musculus |
| Characteristics |
age: E17.5
cell type: EGFP positive retinal cells
genotype: BAC-Crx-EGFP
genetic background: 129Sv/Ev
|
| Treatment protocol |
The mouse retinas were dissected using forceps.
|
| Growth protocol |
Mice were housed in a temperature-controlled room with a 12 h light/dark cycle. Fresh water and rodent diet were available at all times.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (100ng) of the sorted positive or negative cells were isolated using TRIzol reagent (Invitrogen). The whole taranscript cDNA synthesis and amplification kit (Affymetrix) conform to the manufacture’s instruction.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was prepared using IVT labeling kit.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to GeneChip mouse genome 430 2.0 arrayin Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000-7G.
|
| Description |
Gene expression data from sorted EGFP positive cells
|
| Data processing |
Signal intensity was determined using GeneChip Operating Software 1.4. MoEx-1_0-st-v1.r2.pgf. MoEx-1_0-st-v1.r2.dt1.mm8.mps. Quantile normalized gene expression values were obtained with GeneSpring software. The normalization method is RMA algorithm. We adoptated a threshold at signal intensity 20.
|
| |
|
| Submission date |
Nov 24, 2010 |
| Last update date |
Dec 25, 2010 |
| Contact name |
Takahisa Furukawa |
| Organization name |
Osaka Bioscience Institute
|
| Department |
Developmental Biology
|
| Lab |
Furukawa Lab
|
| Street address |
6-2-4 Furuedai
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0874 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE25607 |
Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse. |
|
