|
| Status |
Public on Nov 30, 2013 |
| Title |
Normal endometrium_f |
| Sample type |
RNA |
| |
|
| Source name |
Endometrium
|
| Organism |
Homo sapiens |
| Characteristics |
donor: healthy donor
tissue: endometrium
disease state: Normal (control)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Normal endometrium
f.CEL
|
| Data processing |
Data were analysed with oneChannelGUI (1.16.2) Bioconductor (2.7) package: RMA + quantile normalization
|
| |
|
| Submission date |
Nov 28, 2010 |
| Last update date |
Nov 30, 2013 |
| Contact name |
Raffaele A Calogero |
| E-mail(s) |
raffaele.calogero@unito.it
|
| Phone |
++39 0116706454
|
| Organization name |
University of Torino
|
| Department |
Molecular Biotechnology Center
|
| Lab |
Bioinformatics and Genomics Unit
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
To |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE25628 |
Endometriosis transcription profiling |
|
