|
| Status |
Public on Oct 14, 2022 |
| Title |
im_P5 |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: P5
subset: stromal and hematopoietic cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The bone marrow cell samples were harvested from tibia and femur of P5. The samples were dissociated into single cell suspension and were sorted by Flow cytometry for library preparation.
The libraries were prepared for sequencing using standard 10X 3'v3 protocols
10x 3'v3 single cell RNAseq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The standard celllranger mkfastq software version 3.0 used for basecalling.
The standard celllranger count software version 3.0 used to produced raw expression matrix.
The matrix were normalized and scaled by Seurat v4.0 in R4.0.0 environment.
Assembly: mm10 and rn6
Supplementary files format and content: Matrix
|
| |
|
| Submission date |
Aug 15, 2022 |
| Last update date |
Oct 14, 2022 |
| Contact name |
Guolun Wang |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE184940 |
The integrated single cell RNAseq analysis of bone marrow cells produced by mouse-mouse intraspecies blastocyst complementation and mouse-rat interspecies blastocyst complementation |
|
| Relations |
| BioSample |
SAMN30313236 |
| SRA |
SRX17067618 |
