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Status |
Public on Nov 18, 2022 |
Title |
Non-pupating larvae 5 days 2 |
Sample type |
SRA |
|
|
Source name |
eye-antennal imaginal discs
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Organism |
Drosophila melanogaster |
Characteristics |
tissue: eye-antennal imaginal discs genotype: y w; +/+; +/+ medium: pupation-preventing food time point: 5 days after egg laying group: actively growing
|
Treatment protocol |
The YPH erg2∆::TRP1 yeast strain unable to produce the hormone precursors necessary for the onset of pupation in Drosophila was cultured for 48h at 30°C, centrifuged at 4°C, 1500g for 3min, and the pellet was washed with PBS 3 times as described {Katsuyama, 2013}. The resulting yeast paste was stored at -20°C. For medium preparation, the yeast paste was boiled for 30 minutes and mixed with 10% sucrose, 1.5% agar solution and sterile water in the proportions 10:1:8:20. Nipagin and penicillin were added to prevent fungal and bacterial growth. Adult flies were left overnight to lay eggs, the medium was incubated at 25°C and the larvae were dissected on the day specified.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Drosophila imaginal discs were dissected and transferred into RNAse-free 1xPBS on ice; a minimum of 30 discs per sample were used. RNA was extracted immediately using the RNAeasy mini spin kit (Qiagen) and stored at -80°C. Library preparation was performed according to the QuantSeq 3’ mRNA-Seq Library Prep kit FWD (Lexogen).
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Quality control and trimming of the sequencing reads was performed using FastQC and Cutadapt (-q 15 --minmum-length 60) Trimmed sequence reads were mapped to dm6 using STAR v2.6.1d (with default mapping parameters). Reads were counted with STAR --quantMode GeneCounts Assembly: dm6 Supplementary files format and content: Tab-delimited text file with raw gene counts for every gene and every sample
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Submission date |
Aug 17, 2022 |
Last update date |
Nov 18, 2022 |
Contact name |
Georg Halder |
E-mail(s) |
georg.halder@kuleuven.be
|
Organization name |
VIB-KU Leuven
|
Department |
VIB-KU Leuven Center for Cancer Biology
|
Lab |
Laboratory of Growth Control
|
Street address |
Herestraat 49
|
City |
Leuven |
ZIP/Postal code |
3000 |
Country |
Belgium |
|
|
Platform ID |
GPL25244 |
Series (2) |
GSE211457 |
Hippo signaling instructs ectopic but not normal organ growth [Drosophila NonPupatingLarvae] |
GSE211461 |
Hippo signaling instructs ectopic but not normal organ growth |
|
Relations |
BioSample |
SAMN30370742 |
SRA |
SRX17116488 |