|
Status |
Public on Nov 18, 2022 |
Title |
MARCM sd 1 |
Sample type |
SRA |
|
|
Source name |
eye-antennal imaginal discs
|
Organism |
Drosophila melanogaster |
Characteristics |
tissue: eye-antennal imaginal discs genotype: sd47M FRT19A/tub-Gal80 FRT19A; ey-FLP Act 5C<y+<Gal4 UAS-GFP/+ batch: seq run 1 time point: 5 days after egg laying group: MARCM sd-
|
Growth protocol |
All larvae were raised on standard fly food at 25°C.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Drosophila imaginal discs were dissected and transferred into RNAse-free 1xPBS on ice; a minimum of 30 discs per sample were used. RNA was extracted immediately using the RNAeasy mini spin kit (Qiagen) and stored at -80°C. Library preparation was performed according to the QuantSeq 3’ mRNA-Seq Library Prep kit FWD (Lexogen).
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
MARCM_Counts.txt
|
Data processing |
Quality control and trimming of the sequencing reads was performed using FastQC and Cutadapt (-q 15 --minmum-length 60) Trimmed sequence reads were mapped to dm6 using STAR v2.6.1d (with default mapping parameters). Reads were counted with STAR --quantMode GeneCounts Assembly: dm6 Supplementary files format and content: Tab-delimited text files with raw gene counts for every gene and every sample in a given experiment
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|
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Submission date |
Aug 17, 2022 |
Last update date |
Nov 18, 2022 |
Contact name |
Georg Halder |
E-mail(s) |
georg.halder@kuleuven.be
|
Organization name |
VIB-KU Leuven
|
Department |
VIB-KU Leuven Center for Cancer Biology
|
Lab |
Laboratory of Growth Control
|
Street address |
Herestraat 49
|
City |
Leuven |
ZIP/Postal code |
3000 |
Country |
Belgium |
|
|
Platform ID |
GPL25244 |
Series (2) |
GSE211458 |
Hippo signaling instructs ectopic but not normal organ growth [Drosophila YkiHyper] |
GSE211461 |
Hippo signaling instructs ectopic but not normal organ growth |
|
Relations |
BioSample |
SAMN30370816 |
SRA |
SRX17116745 |