|
| Status |
Public on Jun 10, 2011 |
| Title |
Day5 p53KO MEF OSK-1 |
| Sample type |
RNA |
| |
|
| Source name |
p53-null mouse embryonic fibroblasts, retroviral infection, Day5
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic fibroblast
genotype: p53 null
transduction: OSK
|
| Treatment protocol |
Transduction of indicated genes were done by retroviral infection.
|
| Growth protocol |
MEF were cultivated with 10% FBS/DMEM, passage by Trypsin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Trizol.
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Agilent standard protocol
|
| Description |
Gene expression after 5 days retroviral transduction
|
| Data processing |
Data were analyzed with GeneSpring GX 11. The parameters for normalization are as follows: Data transformation: Set measurement less than 0.01 to 0.01, Per chip: Normalize to 50th percentile
|
| |
|
| Submission date |
Jan 04, 2011 |
| Last update date |
Jun 10, 2011 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7202 |
| Series (2) |
| GSE26429 |
Promotion of Direct Reprogramming by Glis1 |
| GSE26431 |
iPS cell generation |
|
