|
| Status |
Public on Jan 20, 2011 |
| Title |
Biomaterial_BL_003_SI |
| Sample type |
RNA |
| |
|
| Source name |
Endemic Burkitt lymphoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lymph-node biopsy
genotype: African patient
age: Children
|
| Treatment protocol |
Samples were taken at diagnosis from untreated patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner G3000.
|
| Description |
Gene expression data from patient's sample
|
| Data processing |
The data were analyzed with Affymetrix GCOS. Normalization: RMA. Baseline Transformation: Median of all samples.
|
| |
|
| Submission date |
Jan 18, 2011 |
| Last update date |
Jan 20, 2011 |
| Contact name |
pier paolo piccaluga |
| E-mail(s) |
pierpaolo.piccaluga@unibo.it
|
| Phone |
+390516364043
|
| Fax |
+390516364037
|
| Organization name |
University of Bologna
|
| Department |
Hematology and Oncological Sciences "L. and A. Serà gnoli"
|
| Lab |
Molecular Pathology
|
| Street address |
via massraenti 9
|
| City |
Bologna |
| ZIP/Postal code |
40138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26673 |
Expression data from Burkitt lymphoma cases |
|
