|
| Status |
Public on Jan 20, 2011 |
| Title |
CG-4 cells, QKI siRNA transfected, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
CG-4 cells, QKI siRNA transfected
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: transiently transfected CG-4 cells
treatment: QKI knockdown
|
| Treatment protocol |
cells were transfected with lipofectamine 2000
|
| Growth protocol |
CG-4 cells cultured in 30% B104 conditioned medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol and purified with Qiagen RNeasy.
|
| Label |
biotin
|
| Label protocol |
GeneChip Whole Transcript (WT) Sense Target Labeling Assay (Affymetrix) with ribominus protocol
|
| |
|
| Hybridization protocol |
GeneChip Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Fluidics protocols for GeneChip ST Arrays (Affymetrix)
|
| Description |
QKI siRNA transfected cells
|
| Data processing |
CEL files were imported into Partek GS and summarized with RMA with the GC background correction. Winsorized mean was determined for transcripts.
RaEx-1_0-st-v1.r2.pgf
RaEx-1_0-st-v1.r2.dt1.rn3.extended.mps
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Sean Ryder |
| E-mail(s) |
Sean.Ryder@umassmed.edu
|
| Phone |
5088561372
|
| Fax |
5088566464
|
| Organization name |
UMass Medical School
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL6543 |
| Series (1) |
| GSE26716 |
Analysis of gene expression in QKI knockdown CG-4 cells |
|
