|
| Status |
Public on Jul 17, 2024 |
| Title |
DCIS-24T |
| Sample type |
RNA |
| |
|
| Source name |
DCIS
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: DCIS
tumor size: 4 mm
tumor grade: 2
|
| Treatment protocol |
Tissue has been macrodissected and target area was collected for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
48 samples from patients with 40 DCIS and 8 matched normal mammary tissues were macrodissected from FFPE slides and isolated for RNA using Rneasy FFPE kit (Qiagen, #73504) following the manufacture's protocol.
|
| Label |
n/a
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Each target gene of interest was detected using a pair of reporter-capture probes to target a 100 bp sequence. Hybridization between target mRNA and reporter-capture probe pairs was incubated at 65°C for 20 h using CT1000 Touch Thermal Cycler (Bio-Rad, CA) according to manufacturer protocol.
|
| Scan protocol |
The hybridized transcriptome probes were imaged and counted using a digital analyzer for image acquisition and data processing (nCounter Digital Analyzer) as per the manufacturer’s protocol.
|
| Data processing |
nSolver software normalization process
|
| |
|
| Submission date |
Sep 23, 2022 |
| Last update date |
Jul 17, 2024 |
| Contact name |
Justin Wong |
| E-mail(s) |
jwong3@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1155 Pressler St
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL32691 |
| Series (2) |
| GSE214093 |
Epigenetic activation of SOX11 is associated with recurrence and progression of DCIS to invasive breast cancer [Nanostring] |
| GSE214094 |
Epigenetic activation of SOX11 is associated with recurrence and progression of DCIS to invasive breast cancer |
|
