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Status |
Public on Oct 18, 2013 |
Title |
esophageal squamous epithelium_12 |
Sample type |
RNA |
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Source name |
esophageal squamous epithelium
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Organism |
Homo sapiens |
Characteristics |
tissue type: esophageal squamous epithelium
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Treatment protocol |
Laser capture microdissection was performed for all BE and EAC cases. Desired tumor cell or BE areas were selected, cut and collected for total RNA isolation.
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Growth protocol |
Biopsies were taken by upper gastrointestinal endoscopy with biopsy sampling. All samples were snap-frozen in liquid nitrogen and stored at -80°C. Each specimen included in this study was histopathologically approved by Dr. Vieth.
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Extracted molecule |
total RNA |
Extraction protocol |
RNeasy (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
Biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ng total RNA (GeneChip Eukaryotic Target labeling Assays for Expression Analysis - Two Cycle labeling Manual, Affymetrix).
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000. Images were processed using GCOS.
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Data processing |
CEL data files were imported in GeneSpring GX 10.0.2 software (Agilent Technologies, Santa Clara, USA) and pre-processed using the GC-RMA algorithm. Statistical analysis for differentially expressed genes was performed using Welch-test.
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Submission date |
Jan 26, 2011 |
Last update date |
Oct 18, 2013 |
Contact name |
Qing Wang |
E-mail(s) |
wang.qing@mdc-berlin.de
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Phone |
+493094063798
|
Fax |
+493094062846
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Organization name |
Charite-Berlin
|
Department |
Surgical Oncology
|
Street address |
Robert Rössle Str. 10
|
City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
13125 |
Country |
Germany |
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|
Platform ID |
GPL570 |
Series (1) |
GSE26886 |
Gene expression profiling of Barrett's esophagus, adenocarcinoma, esophageal squamous epithelium and squamous cell carcinoma |
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