|
| Status |
Public on Jan 25, 2023 |
| Title |
GD12M_CM1 |
| Sample type |
SRA |
| |
|
| Source name |
Myometrium
|
| Organism |
Sus scrofa |
| Characteristics |
tissue: Myometrium
tissue type: Circular muscle
time: day 12 of pregnancy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the HP Total RNA Kit (R6812, Omega Bio-tek, USA)
A total of 18 RNA libraries were constructed for RNA sequencing using Illumina HiSeq X Ten (PE150) platform according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Sus scrofa genome assembly 11.1
Sequence reads were mapped to the Sus scrofa genome assembly 11.1 with HISAT2 (v2.1.0)
The mapped reads were assembled and count by HTSeq (v0.9.1)
Normalize the readscount in data to TPM
Assembly: Sus scrofa genome assembly 11.1
Supplementary files format and content: RNA-seq_data_TPM file include TPM values for each sample
|
| |
|
| Submission date |
Oct 18, 2022 |
| Last update date |
Jan 25, 2023 |
| Contact name |
Weiwei Wang |
| E-mail(s) |
WeiweiWang@webmail.hzau.edu.cn
|
| Organization name |
Huazhong Agricultural University
|
| Street address |
Hongshan District
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL22918 |
| Series (2) |
| GSE216002 |
Integrating Transcriptomic and ChIP-Seq Reveals Important Regulatory Regions Modulating Gene Expression in Myometrium during Implantation in Pigs [RNA-seq] |
| GSE216440 |
Integrating Transcriptomic and ChIP-Seq Reveals Important Regulatory Regions Modulating Gene Expression in Myometrium during Implantation in Pigs |
|
| Relations |
| BioSample |
SAMN31344039 |
| SRA |
SRX17932981 |
