|
| Status |
Public on Feb 04, 2011 |
| Title |
RO38 |
| Sample type |
RNA |
| |
|
| Source name |
B-lymphoid
|
| Organism |
Mus musculus |
| Characteristics |
strain: SW129/C57BL/6J cells
genotype/variation: c-jun knock out
cell type: B-lymphoid
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol followed by clean up using Qiagen´s RNAeasy mini spin colomn according to manufacturer's instuctions.
|
| Label |
biotin
|
| Label protocol |
RNA (200ng) was reverse transcribed using random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Each sample was hybridized to Affymetrix mouse Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs.
|
| Scan protocol |
Washing and staining of microarrays were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000.
|
| Description |
c-jun knock out
|
| Data processing |
Data were collected using Affymetrix AGCC software. Basic statistical analysis was performed with Flexarray software as follows: Background correction (MAS5.0), array normalization (quantile normalization), probe set summarization technique (Tukey biweight [MAS5.0])
|
| |
|
| Submission date |
Feb 02, 2011 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Gerwin Heller |
| E-mail(s) |
gerwin.heller@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Medicine I
|
| Street address |
Währinger Gürtel 18-20
|
| City |
Wien |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27028 |
C-JUN promotes BCR-ABL induced lymphoid leukemia by inhibiting methylation of the 5´ region of Cdk6 |
|
