|
Status |
Public on Nov 07, 2022 |
Title |
M_10 |
Sample type |
SRA |
|
|
Source name |
muscle
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: muscle strain: Sprague Dawley treatment: MPS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using RNeasy Mini Kit (250) Qiagen#74106. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Use fastp software to clean and filter the original offline data to obtain high-quality sequences (Clean Reads) Use Hisat2 software to compare Clean Reads with reference genes (the reference genome version is GRnor_6.0.100) Stringtie 1.3.3b software was used to compare the results and quantify the transcriptome expression; Normalize with TMM (trimmed mean of M values) algorithm, and finally calculate FPKM (Fragments PerKilobase Million) value of each gene Differential analysis of gene expression between samples/groups using edgeR 3.30.3 software package Assembly: GRnor_6.0.100 Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
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|
|
Submission date |
Nov 02, 2022 |
Last update date |
Nov 07, 2022 |
Contact name |
Liu xue ting |
E-mail(s) |
liuxueting1985@outlook.com
|
Phone |
15245248688
|
Organization name |
China Academy of Chinese Medical Science
|
Street address |
Dongzhimen
|
City |
Beijing |
ZIP/Postal code |
10000 |
Country |
China |
|
|
Platform ID |
GPL25947 |
Series (1) |
GSE217118 |
Intervention effect and mechanism of Tianhe Zhuifeng liquid extract on myofascial pain syndrome |
|
Relations |
BioSample |
SAMN31573949 |
SRA |
SRX18122622 |