|
| Status |
Public on Nov 07, 2022 |
| Title |
M_10 |
| Sample type |
SRA |
| |
|
| Source name |
muscle
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: muscle
strain: Sprague Dawley
treatment: MPS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using RNeasy Mini Kit （250） Qiagen#74106.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Use fastp software to clean and filter the original offline data to obtain high-quality sequences (Clean Reads)
Use Hisat2 software to compare Clean Reads with reference genes (the reference genome version is GRnor_6.0.100)
Stringtie 1.3.3b software was used to compare the results and quantify the transcriptome expression; Normalize with TMM (trimmed mean of M values) algorithm, and finally calculate FPKM (Fragments PerKilobase Million) value of each gene
Differential analysis of gene expression between samples/groups using edgeR 3.30.3 software package
Assembly: GRnor_6.0.100
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
Nov 02, 2022 |
| Last update date |
Nov 07, 2022 |
| Contact name |
Liu xue ting |
| E-mail(s) |
liuxueting1985@outlook.com
|
| Phone |
15245248688
|
| Organization name |
China Academy of Chinese Medical Science
|
| Street address |
Dongzhimen
|
| City |
Beijing |
| ZIP/Postal code |
10000 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE217118 |
Intervention effect and mechanism of Tianhe Zhuifeng liquid extract on myofascial pain syndrome |
|
| Relations |
| BioSample |
SAMN31573949 |
| SRA |
SRX18122622 |
