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| Status |
Public on May 08, 2023 |
| Title |
c-01 |
| Sample type |
SRA |
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| Source name |
OR74A
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| Organism |
Neurospora crassa OR74A |
| Characteristics |
strain: OR74A
genotype: cpc-1 knock out
treatment: 2% glucose in constant light at 25°C , 18h
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
The purity of the sample was determined by NanoPhotometer ® (IMPLEN, CA, USA)；The concentration and integrity of RNA samples were detected by Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA).
Sequencing libraries were generated using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina ® (NR604-01/02) (Annoroad Gene Technology Co., Ltd, Beijing) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
FastQC v0.11.5
trimmomatic v0.39
Hisat2 v2.1.0
Stringtie v1.3.3
DEseq2 v1.16.1
Assembly: Neurospora crassa OR74A
Supplementary files format and content: tpm TPM of all strains
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| Submission date |
Dec 06, 2022 |
| Last update date |
May 08, 2023 |
| Contact name |
Xiaolan Liu |
| E-mail(s) |
liu-xl@im.ac.cn
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| Organization name |
Institute of Microbiology, Chinese Academy of Sciences
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| Street address |
NO.1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platform ID |
GPL32914 |
| Series (1) |
| GSE220169 |
The nutrient-sensing GCN2 signaling pathway is essential for circadian clock function by regulating histone acetylation under amino acid starvation |
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| Relations |
| BioSample |
SAMN32067803 |
| SRA |
SRX18507615 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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