|
| Status |
Public on Dec 24, 2022 |
| Title |
Mycobacterium phage Island3, infection of Mycolicibacterium smegmatis mc155(Butters), 150 mins, bio rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Lehigh University
|
| Organisms |
Mycolicibacterium smegmatis MC2 155; Mycobacterium phage Island3; Mycobacterium phage Butters |
| Characteristics |
treatment: treatment1
stage of_infection: late infection
time: 150 mins
strain: Lehigh University
|
| Treatment protocol |
Samples were taken at early (30 mins) and late (150 mins) of infection
|
| Growth protocol |
Bacteria cells were grown to saturated phase and infected with Island3 at MOI of 1:1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Azenta Life Sciences
Azenta Life Sciences RNA-seq Library Preparation Workflow
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
raw_counts
TPM_values
|
| Data processing |
Azenta Life Sciences Bioinformatics analysis workflow. (Differential Gene Expression and Gene Ontology Analysis)
Assembly: Mycolicibacterium smegmatis mc2155, Mycobacterium phage Island3, Mycobacterium phage Butters
Supplementary files format and content: Text document, raw_counts
Supplementary files format and content: Text document, normalized_counts
Supplementary files format and content: Excel comma separated value, DEGs
|
| |
|
| Submission date |
Dec 20, 2022 |
| Last update date |
Dec 25, 2022 |
| Contact name |
Vassie Ware |
| E-mail(s) |
vcw0@lehigh.edu
|
| Phone |
6107583690
|
| Organization name |
Lehigh University
|
| Department |
Biological Sciences
|
| Lab |
Ware
|
| Street address |
111 research drive
|
| City |
Bethlehem |
| State/province |
PA |
| ZIP/Postal code |
18015 |
| Country |
USA |
| |
|
| Platform ID |
GPL32963 |
| Series (1) |
| GSE221406 |
RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) |
|
| Relations |
| BioSample |
SAMN32329675 |
| SRA |
SRX18786941 |
