|
| Status |
Public on Jun 12, 2024 |
| Title |
Posion exon exclusion in male fly, repeat1 |
| Sample type |
SRA |
| |
|
| Source name |
whole body
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
genotype: w1118; Sf3b3PE-
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNAs from the human and Drosophila cells and tissues were isolated by TRIzol (Sigma) and treated with RNase-free DNase I (Invitrogen). For RT-PCR, reverse transcription was performed using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa) and the obtained cDNA was amplified by 2×Hieff PCR Master Mix (Yeasen). the construction of cDNA libraries and sequencing were performed using Illumina HiseqXten-PE150 by Novogene.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
Male_PE- rep1
|
| Data processing |
The adapter sequences were removed from raw reads by cutadapt (version 1.15).
Clean reads from RNA-seq were quality filtered, and then mapped to the human genome (hg38) and Drosophila melanogaster genome (dm6) by STAR.
Assembly: dm6, hg38
Supplementary files format and content: tab-delimited text files include TPM values for mRNA samples.
|
| |
|
| Submission date |
Jan 18, 2023 |
| Last update date |
Jun 12, 2024 |
| Contact name |
Zhan Ding |
| E-mail(s) |
biodingzhan@gmail.com
|
| Organization name |
CAS Center for Excellence in Molecular Plant Sciences
|
| Street address |
300 Feng Lin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL23702 |
| Series (1) |
| GSE223117 |
Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons |
|
| Relations |
| BioSample |
SAMN32778814 |
| SRA |
SRX19058351 |
