|
Status |
Public on May 01, 2007 |
Title |
FVB_DMBA_12_Spindle_GLAZER |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Mouse reference
|
Organism |
Mus musculus |
Characteristics |
Whole mouse total RNA
|
Biomaterial provider |
Cam Patterson-UNC-CH
|
Extracted molecule |
total RNA |
Label |
Cy3
|
|
|
Channel 2 |
Source name |
FVB_DMBA_12_Spindle
|
Organism |
Mus musculus |
Characteristics |
mammary tumor
|
Biomaterial provider |
Robert Glazer - Georgetown
|
Extracted molecule |
total RNA |
Label |
Cy5
|
|
|
|
Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 4.1 analysis software.
|
Description |
FVB_DMBA_12_Spindle_GLAZER
|
Data processing |
Data for both channels were Lowess-normalized and then log(2) ratio was taken
|
|
|
Submission date |
Aug 11, 2005 |
Last update date |
Jul 17, 2015 |
Contact name |
Charles M. Perou |
E-mail(s) |
cperou@med.unc.edu
|
Organization name |
University of North Carolina at Chapel Hill
|
Department |
Professor of Genetics, and Pathology & Laboratory Medicine; Lineberger Comprehensive Cancer Center
|
Street address |
12-044 Lineberger Comprehensive Cancer Center CB# 7295
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-7264 |
Country |
USA |
|
|
Platform ID |
GPL891 |
Series (3) |
GSE3165 |
Identification of conserved gene expression features across human and murine mammary tumors |
GSE14457 |
Rb deletion in mammary stem/progenitor epithelium induces tumors with features of luminal-B or basal-like breast cancer |
GSE65677 |
The Six1 oncoprotein represses translation of p53 via concomitant regulation of RPL26 and microRNA-27a |
|
Relations |
Affiliated with |
GSE34479 |
Data table header descriptions |
ID_REF |
Spot Reference ID |
VALUE |
same as UNF_VALUE but with flagged values removed |
SPOT |
spot number on array |
CH1_MEAN |
channel 1 mean intensity |
CH1_SD |
standard deviation of channel 1 intensity |
CH1_BKD_MEDIAN |
channel 1 background median intensity |
CH1_BKD_SD |
standard deviation of channel 1 background median intensity |
CH2_MEAN |
channel 2 mean intensity |
CH2_SD |
standard deviation of channel 2 intensity |
CH2_BKD_MEDIAN |
channel 2 background median intensity |
CH2_BKD_SD |
standard deviation of channel 2 background median intensity |
TOT_BPIX |
number of background pixels |
TOT_SPIX |
number of spot pixels |
CH2BN_MEDIAN |
channel 2 normalized background median intensity |
CH2IN_MEAN |
channel 2 normalized mean intensity |
CH1DL_MEAN |
channel 1 Lowess_normalized mean intensity |
CH2DL_MEAN |
channel 2 Lowess_normalized mean intensity |
LOG_RAT2N_MEAN |
log2_ratio of (CH2IN_MEAN - CH2BN_MEDIAN) over (CH1_MEAN - CH1_BKD_MEDIAN), CH2IN_MEAN and CH2BN_MEDIAN are global-normalized intensities |
CORR |
correlation coefficient among pixels |
FLAG |
Spot flag. 0:not flagged; negative:flagged as bad spots; positive:flagged as good spots |
CONTROL |
Y: control gene; N: not control |
UNF_VALUE |
LOG_RAT2L_MEAN; log2_ratio of CH2DL_MEAN over CH1DL_MEAN |