|
| Status |
Public on Aug 01, 2011 |
| Title |
Severe 701 |
| Sample type |
RNA |
| |
|
| Source name |
laser capture CA1 tissue gray matter from formalin fixed, paraffin embedded specimens
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Male
age: 85 years old
disease status: Severe
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion)
|
| Label |
biotin
|
| Label protocol |
50 ng of extracted purified nucleic acid underwent RNA amplification using WT-Ovation FFPE System (NuGen) followed by FL-Ovation cDNA Biotin Module V2 (NuGen) for labeling and microarray (Affymetrix HGU133 v2) hybridization.
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
Severe AD; correlation of the expression of each gene was tested with MiniMental Status Examination (MMSE) and neurofibrillary tangle (NFT) scores
|
| Data processing |
The data were analyzed with Expression Console version 1.1 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Eric M Blalock |
| E-mail(s) |
eric.blalock@uky.edu
|
| Phone |
859-323-8033
|
| Organization name |
University of Kentucky
|
| Department |
Molecular and Biomedical Pharmacology
|
| Lab |
Blalock
|
| Street address |
800 Rose St.
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40475 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28146 |
Microarray analyses of laser-captured hippocampus reveal distinct gray and white matter signatures associated with incipient Alzheimer’s disease |
|
| Relations |
| Affiliated with |
GSM21203 |
| Reanalyzed by |
GSE133349 |
