|
| Status |
Public on Apr 28, 2011 |
| Title |
THP1_Microarray_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
THP-1 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
starting total rna: 500ng
cell line: THP-1
|
| Growth protocol |
THP-1 cells were cultured in RPMI1640 (Invitrogen) supplemented with 10% FBS, Penicillin/Streptomycin (Invitrogen), 10 mM HEPES (Invitrogen), 1 mM sodium pyruvate and 50 µM 2-mercaptoethanol. HeLa cells were cultured in Eagle’s MEM (Invitrogen) supplemented with 10% FBS, 1% NEAA (Invitrogen) and Penicillin/Streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cell lysates were harvested in TRIzol reagent (Invitrogen), total RNA was purified from TRIzol lysates according to manufacturer’s instructions and we used RNase free glycogen (Invitrogen) as carrier to the aqueous phase prior to precipitating the RNA with isopropyl alcohol. The quality of the extracted RNA was confirmed with Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
According to Illumina protocol
|
| |
|
| Hybridization protocol |
According to Illumina protocol
|
| Scan protocol |
According to Illumina protocol
|
| Description |
THP-1 total RNA
|
| Data processing |
Chips scans were processed using Illumina BeadScan and BeadStudio software packages, and summarized data was generated in BeadStudio (version 3.4).
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
Apr 28, 2011 |
| Contact name |
Hideya Kawaji |
| E-mail(s) |
kawaji@gsc.riken.jp
|
| Organization name |
RIKEN
|
| Department |
Omics Science Center
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE28148 |
Unamplified Cap Analysis of Gene Expression on a single molecule sequencer (HeliScopeCAGE) |
|
