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Sample GSM697512 Query DataSets for GSM697512
Status Public on Sep 25, 2011
Title Treated HAND brain 4, biological rep2
Sample type RNA
 
Source name Homo sapiens postmortem brain tissue
Organism Homo sapiens
Characteristics treatment: antiretroviral treated
tissue: postmortem brain
disease state: HIV1 with HIV-1-associated neurocognitive disorders
Extracted molecule total RNA
Extraction protocol RNA was isolated from human brain tissue by RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol.
Label biotin
Label protocol Biotin labeling using NuGen Encore Biotin Module.
 
Hybridization protocol The samples were hybridized to Affymetrix Human Genome 133 plus 2.0 arrays. Washing and staining of all arrays were carried out in the Affymetrix fluidics module as per the manufacturer's protocol.
Scan protocol The detection and quantitation of target hybridization was performed with an Affymetrix GeneChip Scanner.
Description gene expression data for portmortem brain tissue uninfected patients
Data processing The .cel data files generated by the Affymetrix microarray hybridization platform were analyzed by the ArrayAssist software (Stratagene, Santa Clara, CA). Probe level analysis was performed using the Robust Microarray Average (RMA) algorithm.
 
Submission date Mar 24, 2011
Last update date Sep 25, 2011
Contact name Alejandra Borjabad
E-mail(s) ab2612@columbia.edu
Phone 9175574628
Organization name St.Luke's-Roosevelt Hospital Center
Department Medicine
Lab Molecular Virology
Street address 432 West 58th, Street
City New York
State/province NY
ZIP/Postal code 10019
Country USA
 
Platform ID GPL570
Series (1)
GSE28160 Significant Effects of Antiretroviral Therapy on Global Gene Expression in Brain Tissues of Patients with HIV-Associated Neurocognitive Disorders

Data table header descriptions
ID_REF
VALUE RMA normalized signal by ArrayAssist

Data table
ID_REF VALUE
1007_s_at 0.026637077
1053_at 0.19000816
117_at -0.09929943
121_at -0.3967743
1255_g_at -0.024132252
1294_at -0.47464323
1316_at 0.2948389
1320_at -0.16547298
1405_i_at -0.044728756
1431_at 0.060133934
1438_at -0.2503419
1487_at -0.025196075
1494_f_at -0.20389986
1552256_a_at 0.24461174
1552257_a_at 0.14759922
1552258_at 0.05845356
1552261_at -0.20198536
1552263_at 0.011269093
1552264_a_at 0.012011528
1552266_at -0.100922585

Total number of rows: 54675

Table truncated, full table size 1195 Kbytes.




Supplementary file Size Download File type/resource
GSM697512_10063_D2.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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