|
| Status |
Public on Mar 26, 2011 |
| Title |
HIV infected patient, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
jejunal tissue collected by upper endoscopy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: jejunum
disease state: HIV+
|
| Treatment protocol |
treatment with highly active antiretroviral therapy was removed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit was used to extract total RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000
|
| Description |
Gene expression data from jejunal tissue
|
| Data processing |
The data were analyzed with dChip
|
| |
|
| Submission date |
Mar 25, 2011 |
| Last update date |
Mar 26, 2011 |
| Contact name |
Michael George |
| E-mail(s) |
mdgeorge@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
451 Health Sciences Drive
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28177 |
Expression data from HAART interruption in HIV patients |
|
