|
| Status |
Public on Feb 21, 2012 |
| Title |
T1_Pre_OF_10_2A_10B |
| Sample type |
RNA |
| |
|
| Source name |
T1_Pre_Fem_Old_SF2a
|
| Organism |
Homo sapiens |
| Characteristics |
subject: old female 10 (OF_10)
gender: female
age: Old (85±1y)
tissue: Vastus lateralis muscle
training state: Untrained
time point: Basal
fiber type: fast-twitch (MHC IIa)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Homogenize the muscle tissue in TRI reagent and extracted RNA according to manufacturer's protocol, and then purified using Rneasy Micro Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
5ng of total RNA was labeled using NuGen WT-Ovation RNA Amp v2 kit and protocol
|
| |
|
| Hybridization protocol |
Affymetrix Fluidics Station 400
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Apr 05, 2011 |
| Last update date |
Feb 21, 2012 |
| Contact name |
Shawn T. Estrem |
| Organization name |
Eli Lilly and Company
|
| Street address |
Lilly Corporate Center
|
| City |
Indianapolis |
| ZIP/Postal code |
46285 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28392 |
Effects of resistance exercise on the transcriptome in MHC I and MHC IIa muscle fibers of young and old women |
|
