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Sample GSM702445 Query DataSets for GSM702445
Status Public on Feb 21, 2012
Title T1_Pre_Female_Old (81405)
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics age: Old
gender: Female
training state: Untrained
time point: Basal
tissue: Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Homogenize the muscle tissue in TRI reagent and extracted RNA according to manufacturer's protocol, and then purified using Rneasy Micro Kit (Qiagen).
Label biotin
Label protocol 40ng of total RNA was labeled using NuGen WT-Ovation RNA Amp v2 kit and protocol
 
Hybridization protocol Affymetrix Fluidics Station 400
Scan protocol Affymetrix GeneChip Scanner 3000 7G
Description EA08017_81405_AGEX_OF_T1_12_H133+.CHP
EA08017_81405_AGEX_OF_T1_12_H133+.CEL
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Apr 06, 2011
Last update date Feb 21, 2012
Contact name Shawn T. Estrem
Organization name Eli Lilly and Company
Street address Lilly Corporate Center
City Indianapolis
ZIP/Postal code 46285
Country USA
 
Platform ID GPL570
Series (1)
GSE28422 Effects of resistance exercise and resistance training on the skeletal muscle transcriptome in young and old adults

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 6089.24 P 4.42873e-05
AFFX-BioB-M_at 11471.3 P 4.42873e-05
AFFX-BioB-3_at 8335.03 P 4.42873e-05
AFFX-BioC-5_at 19220.2 P 4.42873e-05
AFFX-BioC-3_at 12804.7 P 4.42873e-05
AFFX-BioDn-5_at 40743.3 P 4.42873e-05
AFFX-BioDn-3_at 61403.1 P 4.42873e-05
AFFX-CreX-5_at 128952 P 5.16732e-05
AFFX-CreX-3_at 113561 P 4.42873e-05
AFFX-DapX-5_at 3.46521 A 0.529762
AFFX-DapX-M_at 5.67633 A 0.941556
AFFX-DapX-3_at 51.3952 A 0.5
AFFX-LysX-5_at 27.5975 A 0.185131
AFFX-LysX-M_at 8.1821 A 0.559374
AFFX-LysX-3_at 7.94565 A 0.699425
AFFX-PheX-5_at 3.70564 A 0.891041
AFFX-PheX-M_at 5.13072 A 0.937071
AFFX-PheX-3_at 12.4991 A 0.699394
AFFX-ThrX-5_at 13.477 A 0.617401
AFFX-ThrX-M_at 8.57808 A 0.868639

Total number of rows: 54675

Table truncated, full table size 1659 Kbytes.




Supplementary file Size Download File type/resource
GSM702445.CEL.gz 4.9 Mb (ftp)(http) CEL
GSM702445.CHP.gz 295.2 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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