|
| Status |
Public on Mar 18, 2023 |
| Title |
HCT116 cells, siNRF1-DMSO |
| Sample type |
RNA |
| |
|
| Source name |
HCT116 cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colon
Sex: male
cell line: HCT116
genotype: NRF1 KD
treatment: DMSO
|
| Treatment protocol |
Transfection of siRNA was performed using RNAiMAX (Invitrogen). After 48 h from siRNA transfection, cells were treated with DMSO or 1 µM MG132 (Peptide Institute) for 16 h.
|
| Growth protocol |
HCT116 cells were cultured in DMEM/high glucose medium (Wako) supplemented with 10% FBS (Nichirei Biosciences), 40 µg/ml streptomycin, and 40 units/ml penicillin (Wako). Cells were cultured in 37˚C with 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and purification using ISOGEN II (NIPPON GENE) according to the manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Total RNA was processed with the Ambion WT Expression Kit (Affymetrix) accord- ing to the manufacturers’ instructions. cRNA was then fragmented, labelled using the GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Affymetrix Human Gene 1.0 ST Arrays. GeneChip fluidics station 450 was used for processing of the arrays.
|
| Scan protocol |
Array scanning was performed with the GeneChip scanner 3000-7G.
|
| Data processing |
Images were analyzed with the GeneChip operating software (Affymetrix). Expression console and Transcription analysis console (Affymetrix) were used to identify the gene expression.
RMA expression value derived from Expression Console software
|
| |
|
| Submission date |
Mar 13, 2023 |
| Last update date |
Apr 10, 2023 |
| Contact name |
Akira Kobayashi |
| E-mail(s) |
akobayas@mail.doshisha.ac.jp
|
| Phone |
+81-774-65-6273
|
| Organization name |
Doshisha University
|
| Street address |
1-3 Tatara Miyakodani
|
| City |
Kyotanabe |
| ZIP/Postal code |
610-0394 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE227232 |
Expression data from NRF1 knockdown and/or MG132 treated HCT116 cells |
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